Journal: Journal of Bacteriology

Article Title: Extensive and Genome-Wide Changes in the Transcription Profile of Staphylococcus aureus Induced by Modulating the Transcription of the Cell Wall Synthesis Gene murF

doi: 10.1128/jb.01439-06

Figure Lengend Snippet: FIG. 1. Phenotypic characterization of strain COL and the COLspacmurF conditional mutant grown with the optimal IPTG concentration (100 M) (condition A) and a suboptimal IPTG concentration (2.5 M) (condition B). (a) Growth curves were monitored by OD620, and samples were taken for microarray analysis at mid-exponential phase at the same OD620 value of 0.7, as indicated by the arrows. (b) The cell wall precursor pool composition was determined by reverse-phase high-pressure liquid chromatography (30). Components of peaks 1 and 2 were determined by mass spectrometry. Structure 1, UDP-MurNac-Ala-Glu-Lys; structure 2, UDP-MurNac-Ala-Glu-Lys-Ala-Ala (31). (c) The oxacillin MIC was determined by Etest.

Article Snippet: Cells were harvested at the same optical density in the mid-exponential phase of growth, RNA was prepared, and the number and nature of genes which underwent a change in transcriptional pattern were evaluated using a custom-made Affymetrix DNA microarray.

Techniques: Mutagenesis, Concentration Assay, Microarray, High Performance Liquid Chromatography, Mass Spectrometry

Journal: Gene Expression

Article Title: Distinct Gene Expression Profile of Human Mesenchymal Stem Cells in Comparison to Skin Fibroblasts Employing cDNA Microarray Analysis of 9600 Genes

doi:

Figure Lengend Snippet: VERIFICATION OF GENE ARRAY RESULTS BY REAL-TIME QUANTITATIVE PCR ANALYSIS

Article Snippet: Bone sialoprotein is known as initiator of matrix mineralization and is upregulated during the mineralization phase of osteoblast differentiation ( 27 ). cDNA Microarray We employed a custom-made cDNA microarry chip that was constructed at the Institute for Molecular Biology and Tumor Research Marburg as described previously ( 8 ).

Techniques: Real-time Polymerase Chain Reaction, Gene Expression, Binding Assay

Journal: Gene Expression

Article Title: Distinct Gene Expression Profile of Human Mesenchymal Stem Cells in Comparison to Skin Fibroblasts Employing cDNA Microarray Analysis of 9600 Genes

doi:

Figure Lengend Snippet: VERIFICATION OF GENE ARRAY RESULTS BY REAL-TIME QUANTITATIVE PCR ANALYSIS

Article Snippet: We employed a custom-made cDNA microarry chip that was constructed at the Institute for Molecular Biology and Tumor Research Marburg as described previously ( 8 ).

Techniques: Real-time Polymerase Chain Reaction, Gene Expression, Binding Assay

Journal: PLoS ONE

Article Title: Dysregulation of IFN System Can Lead to Poor Response to Pegylated Interferon and Ribavirin Therapy in Chronic Hepatitis C

doi: 10.1371/journal.pone.0019799

Figure Lengend Snippet: The time frame of liver biopsy, microarray analysis, therapeutic period, observation period after combination therapy, and the judging of clinical outcome is shown.

Article Snippet: In order to provide specific information with less data analysis, we developed a custom-made focused DNA microarray called Genopal (Mitsubishi Rayon, Tokyo, Japan) using genes that target human innate-immunity.

Techniques: Microarray